Journal: Circulation Research

Article Title: Loss of Plasma Proprotein Convertase Subtilisin/Kexin 9 (PCSK9) After Lipoprotein Apheresis

doi: 10.1161/circresaha.113.302655

Figure Lengend Snippet: Figure 1. Lipoprotein apheresis (LA) reduces plasma proprotein convertase subtilisin/kexin 9 (PCSK9) levels. A, Pre- and postapheresis plasma PCSK9 levels in 6 patients with familial hypercholesterolemia (FH). Insert: Representative PCSK9 immunoblot from pre- and post-apheresis plasma. B, Pre- and post-apheresis plasma PCSK9 levels during 3 consecutive treatments in 6 FH patients. C, Correlation between low-density lipoprotein (LDL) reduction and PCSK9 reduction after LA. ***P<0.001.

Article Snippet: Sheep polyclonal antibody toward human PCSK9 was purchased from R&D systems (Minneapolis, MN).

Techniques: Clinical Proteomics, Western Blot

Journal: Circulation Research

Article Title: Loss of Plasma Proprotein Convertase Subtilisin/Kexin 9 (PCSK9) After Lipoprotein Apheresis

doi: 10.1161/circresaha.113.302655

Figure Lengend Snippet: Figure 3. Cholesterol and proprotein convertase subtilisin/kexin 9 (PCSK9) adsorption to a scaled-down dextran sulfate column. A, Percent of cholesterol removed from total plasma and its low-density lipoprotein (LDL) and apoB-free fractions by a scaled-down dextran sulfate column (n=3). B, Percent of PCSK9 removed from plasma, its fractions, and purified GST-tagged PCSK9 by the scaled- down dextran sulfate column (n=3). Insert: PCSK9 immunoblot of GST-tagged eluted fraction from glutathione agarose column of media from HEK293T cells transfected with pcDNA-PCSK9-GST. The presence of a single band confirms the purity of the preparation.

Article Snippet: Sheep polyclonal antibody toward human PCSK9 was purchased from R&D systems (Minneapolis, MN).

Techniques: Adsorption, Clinical Proteomics, Purification, Western Blot, Transfection

Journal: CyTA - Journal of Food

Article Title: Dihydromyricetin promotes LDL metabolism in HepG2 cells through the PCSK9/LDLR pathway

doi: 10.1080/19476337.2023.2252044

Figure Lengend Snippet: Figure 3. DMY inhibited PCSK9 expression in HepG2 cells. (a, b) Western blot analysis showing PCSK9 protein levels in HepG2 cells treated with DMY (5–40 µM) for 24 h. (c) qRT-PCR results showing PCSK9 levels in HepG2 cells treated with DMY (5–40 µM) for 24 h. (d) ELISA results showing PCSK9 content in the cell culture medium with DMY (5–40 µM) for 24 h. The values are expressed as means ± SEM (n = 3). *p < .05 and ***p < .001 compared with the control group. DMY, Dihydromyricetin. PCSK9, proprotein convertase subtilisin/kexin type 9.

Article Snippet: We purchased the rabbit anti-human PCSK9, LDLR, HNF1A, FOXO3, SREBP-1c, SREBP-2, and β-actin primary antibodies from Cell Signaling Technology (USA.).

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Cell Culture, Control

Journal: CyTA - Journal of Food

Article Title: Dihydromyricetin promotes LDL metabolism in HepG2 cells through the PCSK9/LDLR pathway

doi: 10.1080/19476337.2023.2252044

Figure Lengend Snippet: Figure 5. Schematic diagram of the mechanism by which DMY regulates the PCSK9/LDLR pathway. PCSK9, proprotein convertase subtilisin/kexin type 9. LDLR, low-density lipoprotein receptor. HNF1A, hepatocyte nuclear factor 1A. LDL-c, low-density lipoprotein cholesterol.

Article Snippet: We purchased the rabbit anti-human PCSK9, LDLR, HNF1A, FOXO3, SREBP-1c, SREBP-2, and β-actin primary antibodies from Cell Signaling Technology (USA.).

Techniques:

Journal: Antioxidants

Article Title: Selected Soybean Varieties Regulate Hepatic LDL-Cholesterol Homeostasis Depending on Their Glycinin:β-Conglycinin Ratio

doi: 10.3390/antiox12010020

Figure Lengend Snippet: Pearson correlations between the concentration of glycinin and β-conglycinin, and the glycinin: β-conglycinin ratio and the evaluated biochemical and cell culture markers of cholesterol metabolism and LDL oxidation.

Article Snippet: Membranes were incubated overnight at 4 °C with primary mouse antibodies for human p-AMPK T172 (2535, Cell Signaling, Danvers, MA; epitope in T172), AMPK (sc-74461; epitope in amino acids 251–550), SREBP-2 (sc-271616, epitope in amino acids 812–975), HMGCR (sc-271595, epitope in amino acids 589–888), SIRT1 (sc-74504, epitope in amino acids 448–747), p-ACC S78/S80 (sc-271965 epitope in amino acids S78 and S80), ACC (sc-137104, epitope in amino acids 1–76), SREBP-1c (sc-17755, epitope in amino acids 41–200), FASN (sc-48357, epitope in amino acids 2205–2504), LDLR (sc-18823, epitope in amino acids 13–47), and PCSK9 (sc-515082, epitope in amino acids 175–334) purchased in Santa Cruz Biotechnology, unless otherwise stated.

Techniques: Concentration Assay, Cell Culture, Phospho-proteomics, Expressing, Activity Assay

Journal: Antioxidants

Article Title: Selected Soybean Varieties Regulate Hepatic LDL-Cholesterol Homeostasis Depending on Their Glycinin:β-Conglycinin Ratio

doi: 10.3390/antiox12010020

Figure Lengend Snippet: Binding energy ( ΔG ) and constant of interaction ( K i ) between 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), proprotein convertase subtilisin/kexin type 9 (PCSK9), and the peptides identified in the selected soybean varieties digested under gastrointestinal conditions.

Article Snippet: Membranes were incubated overnight at 4 °C with primary mouse antibodies for human p-AMPK T172 (2535, Cell Signaling, Danvers, MA; epitope in T172), AMPK (sc-74461; epitope in amino acids 251–550), SREBP-2 (sc-271616, epitope in amino acids 812–975), HMGCR (sc-271595, epitope in amino acids 589–888), SIRT1 (sc-74504, epitope in amino acids 448–747), p-ACC S78/S80 (sc-271965 epitope in amino acids S78 and S80), ACC (sc-137104, epitope in amino acids 1–76), SREBP-1c (sc-17755, epitope in amino acids 41–200), FASN (sc-48357, epitope in amino acids 2205–2504), LDLR (sc-18823, epitope in amino acids 13–47), and PCSK9 (sc-515082, epitope in amino acids 175–334) purchased in Santa Cruz Biotechnology, unless otherwise stated.

Techniques: Binding Assay

Journal: Antioxidants

Article Title: Selected Soybean Varieties Regulate Hepatic LDL-Cholesterol Homeostasis Depending on Their Glycinin:β-Conglycinin Ratio

doi: 10.3390/antiox12010020

Figure Lengend Snippet: Effect of selected soybean digests (V1, V3, V9, V17, and V18 at 253, 160, 76, 64, and 146 µg protein mL −1 , respectively) and simvastatin (ST, 160 ng mL −1 ) on the secretion of angiopoietin-like 3 (ANGPTL3) ( A ), the activity of lipoprotein lipase (LPL) ( B ), the relative protein expression ( C ) of the LDL receptor (LDLR) ( D ) and proprotein convertase subtilisin/kexin type 9 (PCSK9) ( E ) in free fatty-acid-stimulated HepG2 liver cells (500 µmol L −1 oleic: palmitic acid, 2:1). Representation of the PCSK9-peptide (FEEINKVL) in silico interaction in the LDLR binding site ( F ). LDL uptake in hepatocytes co-treated with FFA and selected soybean varieties digested under gastrointestinal conditions ( G , H ). Results are reported as mean ± SD ( n = 3). Bars with different letters significantly differ according to ANOVA and Tukey’s multiple range test ( p < 0.05).

Article Snippet: Membranes were incubated overnight at 4 °C with primary mouse antibodies for human p-AMPK T172 (2535, Cell Signaling, Danvers, MA; epitope in T172), AMPK (sc-74461; epitope in amino acids 251–550), SREBP-2 (sc-271616, epitope in amino acids 812–975), HMGCR (sc-271595, epitope in amino acids 589–888), SIRT1 (sc-74504, epitope in amino acids 448–747), p-ACC S78/S80 (sc-271965 epitope in amino acids S78 and S80), ACC (sc-137104, epitope in amino acids 1–76), SREBP-1c (sc-17755, epitope in amino acids 41–200), FASN (sc-48357, epitope in amino acids 2205–2504), LDLR (sc-18823, epitope in amino acids 13–47), and PCSK9 (sc-515082, epitope in amino acids 175–334) purchased in Santa Cruz Biotechnology, unless otherwise stated.

Techniques: Activity Assay, Expressing, In Silico, Binding Assay